Skip to main content
. 2020 May 28;23(6):101203. doi: 10.1016/j.isci.2020.101203

Figure 6.

Figure 6

Postsynaptic Deletion of PTPσ Exerts No Effects on Excitatory Synapse Organization in Hippocampal CA1 Region

(A and F) Representative immunofluorescence images of the stratum oriens (SO) and stratum pyramidal (SP) layer of control and Wfs1-PTPσ mice using VGLUT1 (A) or GAD67 (F). Scale bar: 20 μm.

(B and C) Quantification of the density, size, and integrated intensity of VGLUT1-positive synaptic puncta in the SO (B) and SR (C) layers of the hippocampal CA1 region. Data are means ± SEMs (n = 8 mice per group; Mann-Whitney U test).

(D) Representative traces of evoked EPSCs at holding potentials of −70 and +40 mV in control and Wfs1-PTPσ mice.

(E) AMPAR/NMDAR ratios at CA3–CA1 synapses, calculated by dividing the EPSC peak amplitude at 10 ms (−70 mV) by the EPSC amplitude at 130 ms (+40 mV). Data are means ± SEMs (n = 14 cells from 4–5 mice; two-tailed Student's t test).

(G–I) Quantification of the density, size, and integrated intensity of GAD67-positive synaptic puncta in the SO (G), SR (H), and SP (I) layers of the hippocampal CA1 region. Data are means ± SEM (n = 8 mice per group; Mann-Whitney U test).