Skip to main content
. 2020 Jun 5;4(11):2477–2488. doi: 10.1182/bloodadvances.2019001364

Figure 2.

Figure 2.

Immunohistochemical analysis of MF engrafted NSG and MISTRG mice BM. (A) Number of human megakaryocytes (Mega, CD61+) per 4 high power fields (HPF, equaling 1 mm2) in NSG and MISTRG mice for patient (PT) samples with ≥10% hCD45+ (grand median, unpaired Student t test). (B) Representative IHC analysis for hCD61 and Gömöri (stains reticulin fibers) of the BM of an untransplanted MISTRG mouse, a MISTRG mouse transplanted with a healthy donor sample (HD), a NSG and a MISTRG mouse transplanted with sample from a post–essential thrombocythemia patient (PT 12), and the BM biopsy from the same patient (scale bars, 50 µm; original magnification ×400). Arrows point to atypical human MF megakaryocytes. Arrowheads point to atypical human ET megakaryocytes. ns, not significant.