Skip to main content
. 2020 Jun 12;20(1):1–18. doi: 10.3727/105221620X15868728381608

Figure 5.

Figure 5

Effects of paracrine-only interactions between 3T3-J2 fibroblasts and PHH microtissues. Schematics for tested culture models: (a) Only PHHs encapsulated within collagen microtissues (control model); (b) PHH microtissues with 3T3-J2 fibroblasts seeded into a Transwell insert (3T3-J2 Transwell); (c) 3T3-J2 fibroblasts coated onto the surface of the polymerized collagen-based PHH microtissues (3T3-J2 coated, control model). Activities of different CYP450 isoenzymes, (d) CYP3A4, (e) CYP2A6, (f) CYP2C9, (g) CYP1A2 in PHH-only, 3T3-J2 Transwell, and 3T3-J2-coated microtissues. Statistical significance is displayed for 3T3-J2 Transwell microtissues relative to PHH-only microtissues (*p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001). Statistical significance is displayed for 3T3-J2-coated microtissues relative to 3T3-J2 Transwell microtissues (+p ≤ 0.05, ++p ≤ 0.01, +++p ≤ 0.001, ++++p ≤ 0.0001).