Fig. 8.

Similarity of EPN inhibition responses between SmD2 and naïve (Glo) cells. (A) SmD2 expressing cells and control Glo cells were exposed to 50 μM epinine (EPN) and luminescence responses were compared to 20 μM forskolin exposure. *** indicates p < 0.001 according to a Student's t-test. (B) Previously identified hits and in vitro screened NPS-2143 analogs were exposed on SmD2 and Glo cell lines at a 10 μM concentration and fold change EPN responses were calculated compared to DMSO-treated cells. (C) 10⁻¹⁰ to 5.10⁻⁵ M concentration-responses analysis was performed with all hits on SmD2 expressing cells. Error bars indicate SD. (E) Chemical structure of NPS-2143 analogs exhibiting the most potent inhibition of SmD2 EPN-evoked luminescent signals.