Figure 1.

High throughput screening of monocyte attachment to topographically patterned surfaces. a) CD14+ human monocytes were isolated and cultured on polystyrene TopoChips for 3 days in the absence of any exogenous cytokines. Each data point represents the mean ± standard deviation from nine TopoChips tested across five independent donors; dotted line indicates flat planar surface Each TopoUnit was imaged independently analyzed using CellProfiler to determine cell attachment the flat, planar surface had a mean attachment of six cells per TopoUnit indicated by blue dotted line. b) Attachment performance rank order of mean monocyte attachment (per individual TopoUnit) was calculated to compare TopoUnit performance. c) Cells were stained with a plasma membrane dye and counterstained with DAPI to quantify attachment. Representative images of high and low attachment TopoUnits (from five independent donors; scale bar = 20 µm).