Figure 2.

Macrophage attachment is mediated by small circular pillars. A) Macrophage attachment versus total pattern area (µm²) with the size of topographical features categorized as high (blue), medium (green), or low (orange) attachment. Categories of macrophage attachment were determined by cluster analysis using Euclidian distance. Representative composite confocal images of B) low attachment and C) high attachment TopoUnits with inset D) orthogonal views of Z‐stack images of macrophage plasma membrane (green) indicates cellular engulfment of the entire cylindrical pillar feature (also counterstained with DAPI (blue); Scale bar = 10 µm).