Figure 5.

NFATc2 is the downstream target of UCA1/EZH2 complex. A,B) Western blot analysis of NFATc2 protein levels in HepG2 cells transfected with UCA1 siRNA and UCA1 overexpression plasmids in response to indicated concentrations of As (n = 3). C) Relative levels of NFATc2 in HepG2 cells transfected with EZH2 plasmids were detected by qRT‐PCR analysis (n = 3). D) The protein contents of EZH2 and NFATc2 in HepG2 cells pretreated with 1 µmol DNEP at different time points were analyzed by Western blot analysis (n = 3). E) Western blot analysis of the protein contents of NFATc2 in NC siRNA control and NFATc2 siRNA cells exposed to the indicated dosage of As (n = 3). F) Cell cycle distribution in scrambled control and NFATc2 siRNA HepG2 cells in response to As was analyzed via flow cytometry, after staining by PI (n = 3).