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. Author manuscript; available in PMC: 2021 Jun 1.
Published in final edited form as: Metabolism. 2020 Apr 7;107:154225. doi: 10.1016/j.metabol.2020.154225

Figure 4. CR attenuates CL withdrawal-mediated loss of mitochondria in WAT.

Figure 4.

(A) Immunoblots and densitometric analysis of the mitochondrial proteins, and (B) Mitochondrial biogenesis and dynamics and densitometric analysis of the iWAT of WT mice injected with CL (1 mg/kg) for 7 days following which one group of mice was withdrawn from CL injection and maintained for another additional 10 days (n=4–5 mice/group). (C–F) C57BL6 mice fed with 60% high-fat diet for 7 weeks were injected with CL (1 mg/kg) for 7 days with or without 40% CR, and then CL was withdrawn for 10 days while the mice were continued on their respective dietary regimen (CL-WDL or CL-WDL+CR). For the CL group, CL (1 mg/kg) was injected for 7 days in mice fed with high-fat diet ad libitum. (C) Fat mass and weight of organs. (D) H&E analysis (top panel) and TOM20 immunostaining (bottom panel) in iWAT, magnification - 20X. (E) Immunoblots and densitometric analysis of the mitochondrial proteins. (F) Western blot and densitometric analysis of proteins involved in mitochondrial biogenesis and dynamics in the iWAT. Results are representative data from one cohort. Bar graphs are presented as mean SE ± SEM (*p < 0.05, **p < 0.01).