Skip to main content
. 2020 Jun 4;26(6):862–879.e11. doi: 10.1016/j.stem.2020.05.004

Figure 2.

Figure 2

Cardiac Fibroblasts Promote Structural Maturation of hiPSC-CMs in Microtissues

(A) Schematic showing cellular composition of cardiac MT groups. Cell percentages (black) and numbers (gray) are indicated.

(B and C) Representative immunofluorescence images for (B) cardiac sarcomeric proteins TNNI (green) and ACTN2 (red) in MTs (scale bar: 10 μm) and (C) ACTN2 (red) in cells dissociated from MTs (scale bar: 20 μm). Nuclei are stained with DAPI (blue).

(D) Representative transmission electron microscopy (TEM) images showing sarcomeres in different MTs. Scale bar: 1 μm.

(E) TEM images showing caveolae (c), T-tubule like structures (t), Z-lines (Z), I-bands (I), H-zones (H), and elongated mitochondria with complex cristae (red arrows) in CMECFs. Scale bar: 0.5 μm.

(F) Sarcomere organization (sarcomere alignment index; n > 45 areas from 3 MTs per group; ∗p < 0.05) and sarcomere length (n > 47 areas from 3 MTs per group; ∗p < 0.001) from immunofluorescence analysis in MTs from CTRL1 hiPSCs.

(G) Sarcomere length in hiPSC-CMs dissociated from MTs. n > 28 areas from at least 3 independent slides per MT group.

(H) Sarcomere length from TEM in MTs from CTRL1 (n > 41 areas from at least 2 independent stitches per group; ∗p < 0.05). Data are mean ± SEM. One-way ANOVA with Dunnett’s multiple comparisons test is shown.