Fig. 4.

Gch^fl/flTie2cre macrophages stimulated with LPS/IFNγ in DMEM F12 media containing 10% FBS produce NO. (A) Image showing the results of a Griess assay carried out on media supernatants from wild type (WT), iNOS knockout (iNOS KO), Gch^fl/fl and Gch^fl/flTie2cre macrophages following overnight incubation in OptiMEM containing 0.2% BSA or DMEM F12 media containing 10% FBS and stimulation with LPS/IFNγ. The intensity of pink colouring correlates with nitrite levels. (B) A plate reader (absorbance 550 nm) was used to quantify the concentration of nitrites in media on the plate shown above. All data are presented as means ± range. ND – not detectable.