Fig. 5.
Macrophages cultured in MCSF and treated with GMCSF produce more nitric oxide upon stimulation with LPS/IFNγ. (A) NOx (nitrite + nitrate) and (B) nitrites were measured in media supernatants from LPS/IFNγ Gchfl/fl macrophages grown in different growth media. (C) The proportion of nitrite as a percentage of NOx in media. (D) Nitric oxide synthase activity was determined as a percentage of radiolabelled arginine converted into radiolabelled citrulline. (E) Western blot analysis of iNOS protein using β-tubulin as a loading control. (F) Quantification of the abundance of iNOS protein relative to β-tubulin. All data are presented as means ± SEM. Statistics were calculated using 2-way ANOVA and Tukey's post-test (n = 4, *P < 0.05, **P < 0.01, ***P < 0.001).