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iNOS regulation is dependent on the macrophage culture conditions. Western blots were carried out to determine the monomer/dimer ratio and phosphorylation status of the iNOS enzyme. BMDMs were cultured in either LCM, MCSF alone, or MCSF + GMCSF and harvested for Western blot analysis and determination of Monomer/Dimer ratio as an indicator of iNOS activity. Western Blots are representative images of three separate experiments.
