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Biopterin measurements in macrophages cultured in LCM, MCSF and MCSF + GMCSF media. (A) BH4, (B) BH2 and (C) biopterin were measured in Gchfl/fl macrophages grown in different growth media. (D) Total biopterins and (E) the ratio BH4:BH2+B were calculated. All data are presented as means ± SEM. Statistics were calculated using 2-way ANOVA and Tukey's post-test (n = 5, *P < 0.05, **P < 0.01, ***P < 0.001).
