Table 2.
Properties of G3Cgh conjugates: The average molecular weights (MW) were calculated based on the conjugate stoichiometry estimated by 1H-NMR spectroscopy. The values of ζ potential and number- and volume-averaged diameter of conjugate molecules (d(N) and d(V), respectively) were measured at pH 5. The last column shows an averaged number of non-converted primary amine groups of conjugates (n(NH2)).
| Entry | Species | MW (g⋅mol−1) |
Tg (°C) |
ζ (mV) |
d(N) (nm) |
d(V) (nm) |
ν(CO) (cm−1) |
n(NH2) |
|---|---|---|---|---|---|---|---|---|
| 1 | G3 | 6909 | −19.1 | 21.3 | 3.60 | 4.15 | 1634 | 32 |
| 2 | G35gh | 7950 | 5.2 | 7.3 | 3.80 | 4.61 | 1646 | 27 |
| 3 | G310gh | 8991 | 20.2 | 5.6 | 3.80 | 4.73 | 1640 | 22 |
| 4 | G316gh | 10,240 | 22.6 | 3.3 | 3.97 | 4.60 | 1640 | 16 |
| 5 | G318gh | 10,656 | 25.9 | 3.2 | 3.51 | 4.18 | 1643 | 14 |
| 6 | G332gh | 13,587 | 20.0 | 1.1 | 4.00 | 4.60 | 1644 | 0 |
| 7 | G39Cyt11gh | 10,434 | 46.7 | 20.1 | 70.6 | 81.0 | 1646 | 12 |
| 8 | G33Cyt14gh | 10,151 | 26.7 | 5.7 | 4.13 | 4.68 | 1643 | 15 |
| 9 | G32Cyt26gh | 12,539 | 36.4 | 3.3 | 4.34 | 4.77 | 1642 | 4 |
| 10 | G32Cyt23gh | 11,915 | 38.3 | 3.5 | 4.10 | 4.70 | 1643 | 7 |
| 11 | G32Cyt16gh | 10,458 | 10.4 | 7.0 | 4.24 | 4.68 | 1623 | 14 |