Table 2.
Comparison of the proteolytic efficiency of nepenthesin to other proteases used for industrial applications.
| Applications | Proteases | Purification | Substrates | Molar Ratio (Protease: Substrate) | References |
|---|---|---|---|---|---|
| HDX-MS | Porcine pepsin | Reagent grade (Sigma) | XRCC4, XLF, BRCT, PNK, myoglobin, cytochrome C |
1:0.00192 | [47] |
| Nepenthesin II | Crude | XRCC4, XLF, BRCT, PNK, myoglobin, cytochrome C |
1:2.63 | ||
| Celiac treatment | CysProt EP-B2 | Recombinant (E. coli) | α2-gliadin | 1:10 | [68] |
| Nepenthesin II | Crude | α2-gliadin | 1:12000 | [62] |