Figure 2.

Changes in DHA transfer through the trophoblasts exposed to control and GDM conditions. Primary human trophoblasts were cultured in Transwell inserts in standard conditions or with medium containing 25-mmol/L glucose and 10⁻⁷-mol/L insulin for 72 h. Transfer of ¹⁴C-DHA from the apical to the basal chamber was measured using liquid scintillation counting. (a) Time-dependent increase in the transfer of ¹⁴C-DHA (1 μCi/mL) to the basal chamber. * p < 0.05 vs. zero time point. (b) Dose-dependent transfer of ¹⁴C-DHA to the basal chamber after 24 h. * p < 0.05 vs. respective time point in control. Values are expressed as mean ± SD of three independent experiments.