Figure 4.

Changes in mRNA and protein levels of genes related to fatty acid transport in the trophoblasts exposed to control and GDM conditions. Primary human trophoblasts were cultured in standard conditions or with medium containing 25-mmol/L glucose and 10⁻⁷-mol/L insulin for 72 h. (a) Real-time PCR was used to assess mRNA levels, and quantitation of mRNA expression was normalized relative to GAPDH. (b) Western blotting was used for protein quantification. Representative blots for proteins are shown at the lefr; blot densities obtained from densitometric scanning of proteins normalized to β-actin are shown at the right. Values are expressed as mean ± SD of three independent experiments. * p < 0.05 vs. control.