Figure 6.

Effect of SIRT1 on DHA transfer through the trophoblasts exposed to control and GDM conditions. Primary human trophoblasts were cultured in Transwell inserts in standard conditions or with medium containing 25-mmol/L glucose and 10⁻⁷-mol/L insulin for 72 h. Cells cultured in control conditions were treated with SIRT1 inhibitor (EX527, 1 µmol/L) for 24 h. Cells cultured under GDM conditions were treated with recombinant SIRT1 (50 ng/L) or SIRT1 activator (SIRT1729, 1 µmol/L) for 24 h. Transfer of 14C-DHA (1 μCi/mL) from the apical to the basal chamber was measured using liquid scintillation counting. Data are expressed as mean ± SD of three independent experiments. * p < 0.05 vs. vehicle in control conditions. # p < 0.05 vs. vehicle in GDM conditions.