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. 2020 May 11;8(5):706. doi: 10.3390/microorganisms8050706

Figure 4.

Figure 4

Prediction of the structure of A1S_0316 and specific binding of A1S_0316 to the AbOmpA promoter. The structure prediction model of A1S_0316 was obtained by submitting the amino acid sequence of the protein to the SWISS-MODEL program (A). The interaction between the purified A1S_0316 and the AbOmpA promoter (–231 to +100) was shown by gel shift assay (B). In lanes 6–7, “cold DNA” specifies the unlabeled competitor AbOmpA promoter DNA fragments used at 10-fold excess relative to the labeled probe DNA.