Figure 1.

Study timeline relative to aerosol or ID BCG vaccination. Rhesus macaques received BCG vaccination delivered by aerosol (n = 6) or intradermal injection (n = 6) at study week zero or were left as unvaccinated controls (n = 7). All animals received ultra-low dose (ULD) aerosol challenge with M. tuberculosis Erdman strain (ERD ≤ 10 CFU) at study week 21 and were monitored for up to 16 weeks following infection (study week 37). Blue shaded circles represent procedures involving blood sample collection and application of immunological analyses, large open circles represent key study events: vaccination and ULD aerosol M. tuberculosis challenge, open circles indicate application of in vivo CT scanning. All animals were euthanized, and post-mortem (PM) necropsies conducted upon completion of the study schedule (shaded circles).