Figure 7.

Systemic purified protein derivative (PPD)-specific IFN-γ spot forming unit (SFU) frequency measured by ELISpot assay following aerosol or ID Bacille Calmette-Guérin (BCG) vaccination. PPD-specific IFN-γ SFU measured in individual aerosol (A) or ID (B) BCG-vaccinated animals are shown as points with vaccination group median line. Vaccination with aerosol or intradermal BCG is indicated by a dotted line at study week zero. Pre-vaccination SFU frequency is shown as the median of at least 3 replicate values for each animal. Plot C shows a dot plot comparison of IFN-γ SFU frequency four weeks after vaccination, and plot D shows area under the curve (AUC) values calculated from each animal’s SFU frequency profile between weeks 0–20. The group median is indicated by horizontal lines and Mann–Whitney U-test significance values are shown.