Figure 4.

Oral administration of sesamol increases energy expenditure in mice. (A, B) Energy expenditure was evaluated by measurement of oxygen consumption and carbon dioxide production. O₂ consumption (A) and CO₂ production rates (B) of control- and sesamol-treated mice (100 mg/kg) were measured by indirect calorimetry using CLAMS after 3 weeks on the HFD (n = 5 per group). Energy expenditure was measured at 3 weeks of treatments when body weights did not start to diverge. Bar graph (right panel) represents the average of O₂ consumption or CO₂ production in each group. (C) Respiratory exchange ratio (RER) of the control- and sesamol-treated mice (n = 5 per group). (D) Total physical activities of control- and sesamol-treated mice (n = 5 per group). Data represent mean ± s.e.m. and statistically significant differences between the control- and sesamol-treated mice were determined by Student’s t-test (* p < 0.05).