Table 2.

Box–Behnken design matrix with the experimental responses over process parameters in Talaromyces albobiverticillius 30548.

Exp. Run	pH	Temperature (°C)	Agitation Speed (rpm)	Fermentation Time (h)	Orange Pigment Yield (g/L1)	Red Pigment Yield (g/L2)	Dry Biomass Weight (g/L)
1	0	0	0	0	0.30	0.83	3.35
2	0	0	1	−1	1.15	1.44	5.38
3	0	1	0	1	0.30	0.82	1.97
4	−1	0	0	1	0.70	1.08	3.05
5	0	−1	0	−1	0.90	1.23	3.76
6	0	1	1	0	1.10	1.37	6.17
7	−1	0	0	−1	0.65	1.04	2.39
8	0	0	0	0	1.25	1.47	5.47
9	0	0	0	0	0.65	0.95	3.34
10	0	1	−1	0	1.20	1.48	6.05
11	1	1	0	0	0.40	0.85	4.83
12	0	0	0	0	1.40	1.68	5.10
13	0	0	0	0	1.00	1.31	3.33
14	0	−1	−1	0	−0.10	0.43	0.21
15	−1	0	1	0	0.65	1.03	3.75
16	−1	−1	0	0	1.20	1.39	5.34
17	0	1	0	−1	0.10	0.57	2.66
18	1	0	−1	0	1.20	1.48	4.28
19	0	−1	1	0	1.20	1.50	4.61
20	1	0	1	0	1.55	1.73	6.15
21	1	0	0	−1	0.85	1.18	5.21
22	0	−1	0	1	0.70	1.12	2.10
23	0	0	−1	−1	0.75	1.12	3.87
24	1	−1	0	0	0.60	0.98	2.93
25	−1	1	0	0	1.70	1.92	6.67
26	1	0	0	1	1.71	1.92	6.7
27	−1	0	−1	0	1.76	1.93	6.79
28	0	0	1	1	1.77	1.93	7.17
29	0	0	−1	1	1.75	1.93	6.65

1: g/L equiv. quinizarin 2: g/L equiv. RYrp.