Figure 3.

Interferon (IFN)-γ and Interleukin (IL)-2 release from splenocytes treated with Neu peptides. The release of cytokines was assessed in splenocytes collected at sacrifice from treated mice after 96 h stimulation in vitro with Neu peptides. The results show the cytokine release induced by an irrelevant peptide (gag) subtracted from the IFN-γ or IL-2 release of each sample, in two experiments performed in duplicate (*p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001; one-way-ANOVA, Tukey’s multiple comparison). (A,C) show the release of IFN-γ or IL-2 when all peptides were used in the assay, while (B,D) show the contribution of each peptide to the T-cell cytokine release. Results represent three independent experiments of T-cell stimulation with Neu peptides.