Table 3.
Immunoreactivity of rV-neuT-vaccinated BALB-neuT mouse sera with Neu.
| Group | Number of Pooled Sera | Serum Titer (SD) a | p-Value |
|---|---|---|---|
| Corn Oil | 4 | Neg | |
| CUR | 4 | Neg | |
| V-wt | 4 | Neg | |
| V-wt+Corn Oil | 3 | Neg | |
| V-wt+CUR | 4 | Neg | |
| rV-neuT | 6 | 2100 b (±0.012) | 0.000001 c |
| rV-neuT+Corn Oil | 8 | 2400 (±0.020) | 0.00001 c |
| rV-neuT+CUR | 8 | 5000 (±0.019) |
a Immune serum titers of BALB-neuT-vaccinated mice were determined by ELISA against LTR-Neu after NIH3T3 incubation, using pooled sera (T1) at different dilutions (1:100, 1:500, 1:2500, 1:5000). Serum titer represents the mean value of different serum pools ± standard deviation (SD). b Titer was estimated as the highest immune serum dilution generating a specific absorbance of 1.0 at 492 nm. c Differences in titers of the sera was evaluated by a two-tailed Student’s t-test versus rV-neuT+CUR. Neg: negative.