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. 2020 May 9;12(5):1356. doi: 10.3390/nu12051356

Figure 5.

Figure 5

BBB Markers. Immunochemical analysis of the frontal cortex (A,B) and hippocampus (C,D) processed for aquaporin-4 (AQP4) (A,C) and glucose transporter-1 (GLUT1) (B,D). To investigate more than AQP4 and GLUT1 proteins on the same blot, both membranes of the frontal cortex and hippocampus were stripped and incubated with anti-GAPDH antibody (loading control). Control images are re-used for illustrative purposes. The densitometric analysis of bands is expressed as the ratio between the optical density of protein and reference protein (GADPH) where the value for LZRs of different ages is set as 1. Blots are representative of one of three separate experiments. Sections of the frontal cortex of LZRs and OZRs of 12 and 20 weeks of age processed for the immunohistochemistry against AQP4 (E) and GLUT1 (F). Magnification 63X. Calibration bar: 25 μm. Densitometric analysis of the frontal cortex (G,H) and the hippocampus (I,J) processed for AQP4 (G,I) and GLUT1 immunohistochemistry (H,J). Data are expressed in optical density unit (ODU). These are mean + SEM. * p < 0.05 vs. age-matched LZRs.