Table 3.

Mean immunoreaction area of GFAP and Iba-1 in the frontal cortex and hippocampus of lean and obese Zucker rats at the different ages.

	12 Weeks		16 Weeks		20 Weeks
	LZRs (n = 6)	OZRs (n = 6)	LZRs (n = 6)	OZRs (n = 6)	LZRs (n = 6)	OZRs (n = 6)
	Frontal Cortex
Fifth layer Astrocytes immunoreaction area	83.6 ± 5.5	114.8 ± 7.5 *	80.2 ± 5.6	100.1 ± 4.1 *	78.0 ± 4.2	113.3 ± 4.4 *
Fifth layer Soma area of Iba-1 immunoreactive Cells	40.3 ± 2.1	42.4 ± 1.8	39.7 ± 2.8	41.3 ± 1.2	42.5 ± 1.4	47.3 ± 1.7
	Hippocampus
CA1 Astrocytes immunoreaction area	83.9 ± 7.2	105.6 ± 4.5 *	78.7 ± 3.6	109.3 ± 4.8 *	89.6 ± 8.3	114.1 ± 6.1 *
CA1 Soma area of Iba-1 immunoreactive cells	42.0 ± 3.9	41.6 ± 3.1	45.9 ± 4.9	55.1 ± 3.0	45.8 ± 0.8	60.1 ± 1.2 *
CA3 Astrocytes immunoreaction area	83.1 ± 8.7	101.2 ± 5.2 *	93.5 ± 7.0	115.3 ± 6.7 *	101.1 ± 2.7	118.7 ± 2.7 *
CA3 Soma area of Iba-1 immunoreactive Cells	40.7 ± 4.8	40.8 ± 3.1	42.5 ± 2.5	49.1 ± 5.6	46.0 ± 1.1	51.5 ± 2.2 *
Dentate gyrus Mean immunoreaction area	132.7 ± 10.8	130.2 ± 7.1	129.1 ± 8.0	130.4 ± 6.2	135.2 ± 5.5	130.4 ± 7.2
Dentate gyrus Soma area of Iba-1 immunoreactive Cells	40.6 ± 4.9	39.8 ± 2.2	42.2 ± 3.2	50.2 ± 4.9	44.5 ± 2.1	53.4 ± 3.2 *
	Perivascular astrocytes
Total Brain Mean immunoreaction area	150.4 ± 9.3	164.1 ± 9.1	151.4 ± 5.6	140.7 ± 8.2	131.4 ± 6.4	128.7 ± 5.5

For astrocytes, data are expressed as mean immunoreaction area of GFAP positive astrocytes (μm²). For microglial cells data are the area of soma (μm²). Data are the mean ± SEM. * p < 0.05 vs. age-matched LZRs.