Figure 4.

Curcumin or curcumin+TRAIL induced apoptotic cell death. ACHN cells were cultured on six-well plates and exposed to 25 µM curcumin for 4 h. Following this, the cells were incubated with 0.05% DMSO in culture medium or 50 ng/mL TRAIL for 8 or 24 h (a). The treated cells were labelled with YO-PRO-1 (100 µM in DMSO) and propidium iodide (10 µg/mL) immediately prior to flow cytometry analysis. (a) Representative scatter plots show and compare the effects of the treatments at two different time points (8 and 24 h). (b) Results are presented as the mean of the percentage of early apoptosis, late apoptosis, and DNA fragmented cells induced by a specific treatment ± SEM of three independent experiments. *, ** and *** indicate statistically significant difference from respective control at p < 0.05, p < 0.01, and p < 0.001, respectively. Two-way ANOVA was used to analyse the data.