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. 2020 May 1;9(5):92. doi: 10.3390/biology9050092

Figure 6.

Figure 6

The effect of curcumin, TRAIL, and curcumin/TRAIL combination on the expression of pro-and anti-apoptotic target proteins in ACHN cells. Cells were cultured on six-well plates and treated with vehicle control, 50 ng/mL TRAIL, 25 µM curcumin or 50 ng/mL TRAIL/25 µM curcumin combination treatment. After exposure for 8- and 24-h whole cell lysates were prepared with RIPA buffer. Equal amounts of proteins were separated by SDS-PAGE electrophoresis, transferred to nitrocellulose and indirectly probed for (Bax, BCL-2, P53, FADD, cFLIP and CDK1primary antibodies. GAPDH was employed as a loading control. A representative blot is shown from three independent experiments.