Figure 9.

Death receptor 4 upregulation by curcumin/TRAIL combination. (a) ACHN cells were cultured on six-well plates and treated with vehicle control or 25 μM curcumin for 4 h, followed by a further incubation with 50 ng/mL TRAIL for up to 24 h. Cells were incubated with (i) APC- or (ii) PE-conjugated monoclonal anti-human DR4 and DR5 antibodies, respectively. Independent t-test was used to analyse the data. (b) Following cell treatment, RNA was isolated and cDNA was synthesized. qRT-PCR was then performed using a TaqMan-based primer specific to DR4. Results were normalized to GAPDH. The figure is a representative of 3 independent experiments. One-way ANOVA was used to analyse the data.