Figure 12.

CHOP, but not DR4, was activated by JNK following curcumin/TRAIL combination treatment ACHN cells were either pre-treated with DMSO or 10 μM JNK inhibitor (SP600125) for 1 h. Cells were then treated with vehicle control, 50 ng/mL TRAIL, 25 µM curcumin or 50 ng TRAIL/25 μM curcumin. Whole cell protein was extracted at 24 h with RIPA buffer. Equal amounts of protein were separated by SDS-PAGE electrophoresis, transferred to nitrocellulose and indirectly probed for (a) P-JNK, (b) CHOP and (c) DR4 using a mAb and ECL detection system. GAPDH was used as a loading control. Representative blots are shown from 3 independent experiments. ** = p < 0.01 and *** = p < 0.001. One-way ANOVA was used to analyse the data.