Skip to main content
. 2020 Apr 27;8(5):636. doi: 10.3390/microorganisms8050636

Figure 6.

Figure 6

Thymol protects A549 cells against aerolysin-mediated cell injury. For live/dead assay, cells were stained with a LIVE/DEAD Viability/Cytotoxicity Kit and images were captured by a fluorescence microscope. A fluorescent-red dye stained dead cells, while a fluorescent-green dye stained live cells. For lactate dehydrogenase (LDH) assays, LDH release was detected by a Cytotoxicity Detection Kit. (A) untreated cells; (B) cells treated with bacterial supernatant without thymol; (C) cells treated with supernatant of A. hydrophila co-cultured with 16 μg/mL thymol; (D) LDH release of A549 cells when treated with supernatants co-cultured with indicated concentrations of thymol. All data are presented as mean value ± SD of three independent assays.