Figure 1.

pPLAIIIγ-KO, its complementation (COM) and response to different NaCl concentrations; (a) T-DNA insertion site in pPLAIIIγ. Solid boxes indicate exons, thin lines indicate introns, gray boxes represent the untranslated regions (UTR) at both the 5′ and 3′ positions. Arrows (LP, BP and RP) show positions of primers used for PCR. F and R present positions of JLP053 and JLP054 primers, respectively, for pPLAIIIγ relative expression in qPCR; (b) PCR-confirmation of T-DNA insertion in pPLAIIIγ using genomic DNA; (c) relative gene expression of pPLAIIIγ in WT, KO, OE and COM lines by qPCR. UBQ10 was used as internal control. Data are means ± SE (n = 3); (d) representative images of seedlings grown on half-strength MS media supplemented with 0, 75 and 100-mM NaCl. Measurement of primary root length (e) and dry weight (f) of WT, KO and COM lines. Seedlings were grown for 7 days under different treatments after transferring and then measured. Values are mean ± SE (n = 45–60 for root length or 3 biologic repeats for dry weight. One and two asterisks indicate significant differences from WT by Student’s t-test at p = 0.05 and p = 0.01, respectively.