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. 2020 May 20;9(5):650. doi: 10.3390/plants9050650

Figure 3.

Figure 3

Opposite effects of pPLAIIIγ-KO and -OE lines on tolerance to NaCl and PEG; (a) Immunoblotting of GFP-His-tagged pPLAIIIγ in Arabidopsis. Five independent homozygous lines of pPLAIIIγ-OE mutants were examined; (b) confocal images of epidermal cells of WT and pPLAIIIγ-OE:GFP leaves and chlorophyll fluorescence versus GFP; (c) plasmolysis of root epidermal cell of the pPLAIIIγ-OE:GFP. Representative images of WT, KO and OE lines without (d) or with (e) NaCl stress; (f) relative growth of root length of WT, KO and OE lines. Values are mean ± SE (n = 45–60); (g) relative growth of dry weight of WT, KO and OE lines. Values are mean ± SE from three biologic repeats for each line (20 seedlings dry weight per biologic repeat). One and two asterisks indicate significant differences from WT by Student’s t-test at p = 0.05 and p = 0.01, respectively.