Figure 2.

Cocultivation of OvCa cells with PTX-ADSCs kills in 2D and 3D tumor cells and overcomes PTX-resistance. (A) OvCa cells (1 × 10³) were cultured with increasing number of ADSCs and PTX-ADSCs (2D model). After 7 d, cell viability was evaluated by MTT assay (percent of control). * p < 0.05 vs. no ADSCs, one-way ANOVA and Dunnett’s test. (B) Number of PTX-ADSCs capable of reducing 50% of viable cells (MTT assay). Results are means and SDs of three experiments. (C) SKOV3-MCTSs were cultured under non-adherent conditions (3D model) with increasing numbers of ADSCs and PTX-ADSCs (heterospheroids). After 72 h cell viability was evaluated by AnnexinV/PI staining (% of control). Viable cells: AnnexinV/PI negative cells. Results represent the % of control (SKOV3-MCTSs) cultured without ADSCs. Results are means and SDs of three experiments. * p < 0.05 vs. no ADSCs, one-way ANOVA and Dunnett’s test.