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. 2020 Apr 27;12(5):401. doi: 10.3390/pharmaceutics12050401

Figure 7.

Figure 7

Inhibition of SKOV3-MCTS and OVCAR8-MCTS migration/dissemination onto matrix protein coating by PTX-ADSC-CM. (A–D) SKOV3 and OVCAR8 single spheroids were placed onto collagen-I coated plates in the presence of PTX (0–5 ng/mL) and PTX-ADSC-CM (equivalent amounts of PTX). After 48 h, OvCa cells were fixed with methanol and stained with crystal violet. (A,C) Bar charts showing the migration/dissemination rate of spheroids, evaluated as the area covered by migrating cells from spheroids and represented as fold increase respect to the area (pixel) covered at time = 0. Values in the bar graph represent the means ± SDs of three different experiments. * p < 0.05 free PTX vs. medium, ° p < 0.05 PTX-ADSC-CM vs. medium, # p < 0.05 PTX-ADSC-CM vs. free PTX, one-way ANOVA followed by Bonferroni correction. (B,D) Images were captured using an inverted microscope (phase contrast microphotographs, original magnification 4×). Dotted line indicates cell-covered area.