Aerenchyma formation under aerated or stagnant conditions. (a) Cross-sections at 30 mm from the tips of adventitious roots of the wild-type (WT) and iaa13 mutant. Aerenchyma is indicated by magenta arrowheads. Bars = 100 μm. (b,c) Percentages of aerenchyma in root cross-sectional area at 10, 20, 30, 40, and 50 mm. Twenty-day-old aerobically grown WT (b) and iaa13 (c) seedlings were further grown under aerated or stagnant conditions for 48 h. (b,c) Significant differences between the conditions at p < 0.01 are denoted by ** (two-sample t-test). Boxplots show the median (horizontal lines), 25th to 75th percentiles (edges of the boxes), minimum to maximum (edges of the whiskers), and mean values (dots in the boxes) (n = 6).