Figure 2.

Aerenchyma formation under aerated conditions with or without 1-aminocyclopropane-1-carboxylic acid (ACC) treatment. (a) Cross-sections at 30 mm from the tips of adventitious roots of the wild type (WT) and iaa13 mutant. Aerenchyma is indicated by magenta arrowheads. Bars = 100 μm. (b,c) Percentages of aerenchyma in root cross-sectional area at 10, 20, 30, 40, and 50 mm. Twenty-day-old aerobically grown WT (b) and iaa13 (c) seedlings were further grown under aerated conditions with or without 10 µM ACC for 48 h. (b,c) Significant differences between the conditions at p < 0.05 and p < 0.01 are denoted by * and **, respectively (two-sample t-test). Boxplots show the median (horizontal lines), 25th to 75th percentiles (edges of the boxes), minimum to maximum (edges of the whiskers) and mean values (dots in the boxes) (n = 6).