Figure 3.

Workflow showing green fluorescence imaging to identify regions of root tissues with highest levels of root colonization by RAM10, H. seropedicae, bacteria. Isolated 4-cm portions of root were subjected to sonication in saline solution to mechanically remove bacteria. The extract was re-imaged using the GFP signature of the microorganisms to quantify bacteria content in the liquid extract. Background corrections were made in each set of measurements using a deionized water sample. Non-inoculated control roots exhibited a trace amount of fluorescence signal attributable to naturally fluorescent plant metabolites.