Skip to main content
. 2020 May 18;12(5):459. doi: 10.3390/pharmaceutics12050459

Figure 7.

Figure 7

Effect of pirfenidone, galunisertib, and imatinib on extracellular deposition of collagen type I in human renal fibroblasts (HRFs). HRFs were stimulated with macromolecular crowder PVP-40 and TGFβ, and cultured in the presence of pirfenidone (0.5, 1, and 2.5 mM), galunisertib (0.1, 1, and 5 µM), or imatinib (1, 5, and 10 µM) for 48 and 96 h. Representative photomicrographs of immunocytochemistry for extracellular collagen type I fibrils (green) in HRFs treated with compounds for 48 h (a) and 96 h (b), with respective computerized quantitative analyses. Nuclei (blue); scale bars are 100 µm. Data are expressed as mean ± SEM, n = 3; * p < 0.05.