Table 1.
Differential phenotypic characteristics of strain 03113T and closely related strains in the genus Bacillus.
| Characteristic | 1 | 2 | 3 | 4 |
|---|---|---|---|---|
| Optimal growth conditions | ||||
| Temperature for growth (°C) | 37 | 30–37 | 35 | 30 |
| pH for growth | 8.0 | 7.0 | 9.0 | 9.0 |
| NaCl concentration for growth (%, w/v) | 5 | 1 | 0 | 4 |
| The Acid produced from (API 50CH) | ||||
| L-arabinose | + | + | + | − |
| Esculin | + | + | − | − |
| API 20NE | ||||
| β-galactosidase | + | + | − | − |
| Lysine | + | − | − | − |
| Lohn gelatin | + | + | + | − |
| Utilization (Biolog GEN III) | ||||
| dextrin | + | + | − | + |
| d-maltos, d-trehalose, sucrose, d-turanose, d-raffinose | + | − | − | − |
| N-acetyl-d-glucosamine | − | + | + | − |
| N-acetyl-β-d-mannosamine, acetic acid | − | + | − | − |
| Stachyose, d-mannose | w | − | − | − |
| N-acetyl-d-galactosamine | − | w | − | − |
| α-d-glucose | + | − | w | − |
| d-fructose | + | + | w | − |
| Inosine, d-serine, glycerol, d-glucose-6-PO4, d-fructose-6-PO4, nalidixic acid, lithium chloride, aztreonam, lincomycin | − | + | + | + |
| Troleandomycin | − | − | + | − |
| L-aspartic acid, L-glutamic acid, L-histidine, L-pyroglutamic acid, L-serine, L-lactic acid, sodium butyrate | - | + | + | − |
Strains: 1, 03113T; 2, B. dabaoshanensis CCTCC AB 2013260T; 3, B. solani CCTCC AB 2014277T; 4, B. praedii CCTCC AB 2015208T. All strains were negative for sodium thiosulfate, tryptophan, d-cellobiose, gentiobiose, α-d-lactose, d-melibiose, β-methyl-d-glucoside, d-salicin, N-acetyl neuraminic acid, d-galactose, 3-methyl glucose, L-fucose, L-rhamnose, fusidic acid, d-sorbitol, d-mannitol, myo-inositol, d-aspartic acid, minocycline, pectin, d-galacturonic acid, glucuronamide, mucic acid, quinic acid, p-hydroxy-phenylacetic acid, citric acid, α-keto-glutaric acid, d-malic acid, γ-amino-butryric acid, α-hydroxy-butyric acid, β-hydroxy-D, L-butyric acid, α-keto-butyric acid, propionic acid, and formic acid. All strains were positive for sodium lactate and potassium tellurite. All data were from the present study. +, positive; w, weakly positive; −, negative.