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. 2020 Apr 28;9(5):91. doi: 10.3390/biology9050091

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Analysis of the potential implication of the VSR p37 in rDNA promoter hypomethylation in N. benthamiana plants. (A) Differential DNA methylation levels between mock (green) and p37 (red) agroinfiltrated plants in the CG, CHG, and CHH sequence contexts. Error bars depict the standard deviations from two independent biological replicates. (B) RT-qPCR to estimate the relative expression levels of the genes involved in methylation/demethylation with imbalances in PLPV-infected plants. Data are representative of three biological replicates. The statistical significance was tested using a paired t-test (* p < 0.05; ** p < 0.01).