Figure 3.

Biocontrol efficacy of antagonistic bacterial strains against soft rot disease on dicotyledonous carrot (A), potato (B) and Chinese cabbage (C). Both MS2 and antagonistic bacteria were grown in lysogeny broth (LB) medium till OD₆₀₀ ≈ 1.8. One microliter of LB, LB+antagonistic bacteria, LB+MS2 and MS2+antagonistic bacterium was respectively spotted onto the center of tissue slices. The rotting area was measured by Image J 1.52a, and the data were subjected to unpaired two-tailed t-test analysis by Graphpad Prism 8.4.1 (GraphPad Software, San Diego, CA, USA) (ns: no statistical significance, *** p < 0.001). The marks in the plot area represent the diseased area on 10 inoculated plant slices, and the red lines represent the average values of diseased area.