Figure 5.

Biocontrol efficacy of strain 3-10 against banana wilt caused by Fusarium oxysporum f. sp. cubensis FOC4. Strain 3-10 was cultured in LB medium overnight and resuspended with ddH₂O to a final concentration of OD₆₀₀ = 0.1. FOC4 strain was cultured on PDA plates for about 5 days and washed with ddH₂O to collect hypha and conidia. Half roots of each banana seedling (30 seedlings) were randomly cut off, 20 seedlings were soaked in pure FOC4 suspension and 10 were soaked in ddH₂O for 20 minutes, and re-planted in sterilized pots. Then 100 mL of 3-10 cell suspension and ddH₂O was respectively drenched into soils of each 10 FOC4-soaked seedlings, while 100 mL of ddH₂O was also drenched into the soils of the ddH₂O-soaked seedlings. Symptoms were observed every day and pictures were taken in the 16^th day after inoculation. Data were subjected to unpaired two-tailed t-test analysis by Graphpad Prism 8.4.1 (*** p < 0.001). The marks in the plot area represent the virulence scores of the inoculated seedlings, and the red lines represent the average virulence scores.