Figure 4.

Heatmap visualization of protein regulation required for toluene and benzoate degradation pathways in anaerobic denitrifying and aerobic cultures of Magnetospirillum sp. 15-1. Up-regulated proteins are represented by n ≥ 1 and are coloured in shades of red. Down-regulated proteins are represented by n ≤ −1 and are coloured in shades of blue. AN_T represents cultures growing anaerobically with toluene and either 2.5 mM (AN_T2.5), 5 mM (AN_T5), or 10 mM (AN_T10) of KNO₃. AB_T represents cultures growing aerobically with benzoate and supplied additionally with either toluene alone (AB_T) or toluene and 5 mM of KNO₃ (AB_TN). BssD, benzylsuccinate activating enzyme; BssABC, benzylsuccinate synthase; BssE, putative chaperone; BssG-J and BssL, unknown function; BssK, mRNA binding protein; XylR, σ⁵⁴-dependent regulator, TdiC, regulator of TdiR activity; TdiR, toluene degradation regulator; TdiS, toluene degradation inducing sensor; BbsEF, (3-methyl)benzylsuccinate CoA transferase; BbsG, (3-methyl)benzylsuccinyl-CoA dehydrogenase; BbsH, (3-methyl)phenylitaconyl-CoA hydratase; BbsCD, 2-[hydroxyphenyl(methyl)]-succinyl-CoA dehydrogenase; BbsAB, (3-methyl)benzoylsuccinyl-CoA thiolase; BclA, benzoate-CoA ligase; KorBA, 2-oxoglutarate oxidoreductase; BcrABCD, benzoyl-CoA reductase; Fdx, 4Fe-4S ferredoxin; Dch, dienoyl-CoA hydratase; Had, 6-hydroxycyclohex-1-ene-1-carboxyl-CoA dehydrogenase; Oah, 6-oxocyclohex-1-enecarboxyl-CoA hydrolase; NarGHI, nitrate reductase; NarJ protein assembly; NorCB, nitric oxide reductase; NorQ and NorD, unknown function; NirN, haem d1 insertion protein; NirJHLD, unknown functions; NirF, membrane attached lipoprotein; NirS, nitrite reductase; NosZ, nitrous oxide reductase; NosD, putative Cu insertase; NosF, ATP-hydrolyzing of ABC transporters.