Figure 11.

RNA interference by anti-GFP small interfering RNA that was delivered by PLL/Hep IPECs ([Lys–NH₃⁺]:[siRNA–PO₄⁻]:[Hep–SO₃⁻]:[linker] = 1:1:4:0.8) in NIH-3T3 cell line. (A) Fluorescence microscopy images of NIH-3T3 cells after transfection with anti-GFP siRNA complexed by IPECs: a—intact cells; b—cells incubated with cross-linked anti-GFP-siRNA/IPECs; c—cells incubated with cross-linked anti-GFP-siRNA/IPECs and subjected to the treatment 5W UV 365 nm LED lamp for 30 min; d—cells incubated with anti-GFP-siRNA/PEI polyplex. (B) Flow cytometry detection of GFP fluorescence after co-incubation with anti-GFP siRNA containing complexes. Co-incubation time was 4 h. Mean (± SD), n = 5.