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. 2020 May 20;8(5):763. doi: 10.3390/microorganisms8050763

Table 1.

Commercial DNA extraction and purification kits used in this study.

Commercial Kit Manufacturer Cell Disruption Method *
DNA MinipPrep kit ZymoBIOMICS BB/ SB
DNeasy Blood and Tissue kit Qiagen PL + CL/ SB
DNeasy PowerSoil kit Qiagen BB/ SB
DNeasy Ultra Clean Microbial kit Qiagen BB/ SB
Echolution Tissue DNA Micro kit BioECHO PL + CL/ SB
GenElute Bacterial Genomic DNA kit Sigma Aldrich PL + CL/ SB
In-house protocol, based on DNA Investigator kit Qiagen BB/ SB
innu PREP DNA Mini kit Analytik Jena PL + CL/ SB
MagJet Genomic DNA kit Thermo Scientific PL + CL/ MP
MasterPure Complete DNA & RNA Purification kit Lucigen PL + CL/ SP
nexttec 1-step DNA isolation kit for Bacteria Biozym PL + CL/ SB
NucleoSpin Microbial DNA Mini kit Machery & Nagel BB/ SB
PureLink Microbiome DNA Purification kit Thermo Scientific BB/ SB
QIAamp Cador pathogen Mini kit Qiagen PL + CL/ SB
QIAamp DNA Mini kit Qiagen PL + CL/ SB
QIAamp UCP Pathogen Mini kit Qiagen BB/ SB
QIAmp DNA Microbiome kit Qiagen BB/ SB
RTP Bacteria DNA Mini kit Stratec Molecular PL + CL/ SB
smart DNA prep Analytik Jena CL/ MP
Wizard Genomic DNA Purification kit Promega PL + CL/ SP

* PL physical lysis; CL chemical lysis; BB bead-beating; MP magnetic particle based technology; SB silica based technology; SP salt precipitation.