Table 3.
Characteristics of the 20 commercial DNA extraction kits compared.
| No. | Commercial Kit | Cell Disruption Method * | Completion Time ** [min] | Average Cost *** [€/Sample] |
|---|---|---|---|---|
| 1 | MasterPure Complete DNA and RNA Purification kit | PL + CL/SP | 90 | 2.23 |
| 2 | In-house protocol, based on DNA Investigator kit | BB/ SB | 180 | 6.13 |
| 3 | innu PREP DNA Mini kit | PL + CL/SB | 90 | 2.44 |
| 4 | GenElute Bacterial Genomic DNA kit | PL + CL/SB | 90 | 3.08 |
| 5 | DNeasy Ultra Clean Microbial kit | BB/ SB | 50 | 2.79 |
| 6 | QIAamp DNA Mini kit | PL + CL/SB | 150 | 3.90 |
| 7 | NucleoSpin Microbial DNA | BB/ SB | 40 | 3.50 |
| 8 | DNeasy Blood and Tissue kit | PL + CL/SB | 90 | 3.74 |
| 9 | MagJet Genomic DNA kit | PL + CL/MP | 150 | 3.06 |
| 10 | PureLink Microbiome DNA Purification kit | BB/SB | 60 | 6.64 |
| 11 | Wizard Genomic DNA Purification kit | PL + CL/SP | 180 | 2.49 |
| 12 | QIAamp Cador pathogen Mini kit | PL + CL/SB | 180 | 6.24 |
| 13 | DNA Mini Prep | BB/SB | 60 | 6.52 |
| 14 | DNeasy PowerSoil kit | BB/SB | 50 | 6.43 |
| 15 | nexttec 1-step DNA isolation kit for Bacteria | PL + CL/SB | 60 | 2.69 |
| 16 | QIAamp UCP Pathogen Mini kit | BB/SB | 60 | 4.86 |
| 17 | RTP Bacteria DNA Mini kit | PL + CL/SB | 75 | 3.89 |
| 18 | smart DNA prep | CL/MP | 150 | 3.40 |
| 19 | Echolution Tissue DNA Micro kit | PL + CL/SB | 60 | 3.42 |
| 20 | QIAmp DNA Microbiome kit | BB/ SB | 160 | 16.97 |
* PL physical lysis; CL chemical lysis; BB bead-beating; MP magnetic particle based technology; SB silica based technology; SP salt precipitation; ** Approximate time to complete DNA extraction from four samples; *** List price on manufacturer web page accessed April 2020 excluding shipping costs.