Table 5.
A combination of mechanical disruption by bead-beating and DNA extraction with the MasterPure kit resulted in the highest DNA extraction efficiency for B. anthracis spores. Ct: cycle threshold.
| Sample | Concentration Spores [CFU] | Ct Values |
|---|---|---|
| Suspension of untreated B. anthracis spores (control) | 106 | 34.08 ± 0.01 |
| QIAmp DNA Mini kit | 106 | 28.30 ± 0.23 |
| In-house protocol, based on DNA Investigator kit | 106 | 24.76 ± 1.02 |
| MasterPure kit, lysis supernatant | 106 | 25.99 ± 0.10 |
| MasterPure kit, debris pellet | 106 | 25.40 ± 0.30 |
| In-house protocol, based on DNA Investigator kit | 103 | 36.87 ± 1.10 |
| MasterPure kit (lysis supernatant + debris pellet) * | 103 | 34.01 ± 0.97 |
* DNA extracted from the debris pellet and DNA isolated from lysis supernatant are each dissolved in 25 µL TE buffer and pooled. Data represent the mean (±standard deviation, SD) of three independent experiments, each performed at least in triplicate.