Figure 2.

Naegleria fowleri trophozoites incubated with IC₉₀ of STS for 24 h (D–F). Negative control (A–C). Hoechst stain is different in control cells, where no fluorescence is observed (B), and in treated cells, where the nuclei are intense blue (E). Red fluorescence corresponds to the propidium iodide stain (C,F). Visible channel (A,D); Hoechst channel (B,E), and propidium iodide channel (C,F). Images (40×) are representative of the cell population observed in the performed experiments. Images were obtained using an EVOS FL Cell Imaging System AMF4300, Life Technologies, USA. Obtained results were similar for both N. fowleri type strains.